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Agilent technologies
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Image Search Results
Journal: Scientific Reports
Article Title: Tryptophan hydroxylase (TRH) loss of function mutations in Daphnia deregulated growth, energetic, serotoninergic and arachidonic acid metabolic signalling pathways
doi: 10.1038/s41598-019-39987-5
Figure Lengend Snippet: Analysis of microarray results. ( A ) Heat map and Pearson hierarchical clustering of the 147 transcripts identified as underrepresented in CRISP-Cas9 TRH clonal mutants relative to the wild type clone across the four individual-replicates per clone. ( B ) Venn diagram showing the overlapping of transcriptomic effects between individuals of the three mutant clones. T+, TA−,TB− refer to one mono-allelic and to two bi-allelic TRH mutant clones, respectively.
Article Snippet: The
Techniques: Microarray, Mutagenesis, Clone Assay
Journal: Scientific Reports
Article Title: Tryptophan hydroxylase (TRH) loss of function mutations in Daphnia deregulated growth, energetic, serotoninergic and arachidonic acid metabolic signalling pathways
doi: 10.1038/s41598-019-39987-5
Figure Lengend Snippet: Schematic representation of the metabolic/physiological intercorrelation of identified de-regulated genes in Bi-allelic mutated TRH clones. The scheme represents both the pre- (left) and the post-synaptic (right) neuron of a putative serotoninergic synapsis ( A ) and the insulin signalling pathways ( B ). Outlined pathways are adapted from Heckman et al . , Boucher et al . , Edgar and Tootle and Spradling ones. De-regulated Genes/enzymes/neurotransmiters are shown in red (down) or green (up) boxes, those identified as de-regulated in the microarray but not confirmed by qPCR are depicted in white boxes.
Article Snippet: The
Techniques: Clone Assay, Microarray